The implementation of analytical techniques has revolutionized control of cell cultures during bioprocess manufacturing. The parameters used for monitoring cell cultures can be classified into four categories: cellular physiology, cellular environment, cellular metabolites and contaminant monitoring.
Undoubtedly, all these techniques provide us with plenty of information about cell cultures. However, are they enough to understand the behavior of cells in their complex environment?
Example: Cell lines should give consistent productivity and product quality throughout the production process. Many factors such as shear stress may affect cell proliferation and activate genes associated with apoptosis (Bak, Bax, Caspase 3). Hence, the identification of genes expression level will help us to retro control cell culture by adding anti apoptotic proteins.
As for cell proliferation / apoptosis, gene expression level for genes implicated in metabolism and other signaling pathways can be monitored at GenSensor.
We help you defining biomarker panel adapted to your bioproduction, so you understand the modifications in gene expression level at different time points.
You’ll understand how your cells survive and function properly in the complex and noisy environment, and how they survive and behave appropriately with a complex transcriptome.
